![]() These strips are enclosed in a glassine envelope and processed through the sterilizer. We biologically monitor our sterilizer once a week to ensure complete sterilization using spore strips and keep accurate records for our monitoring. Of the three methods, biologic monitoring is regarded as the most valid for monitoring the sterilization process, for it uses live, highly resistant bacterial spores. Using bacterial spores to monitor the sterilization process is referred to as biologic monitoring (or spore-testing), and the bacterial spores used for monitoring the sterilization process are referred to as biologic indicators (BIs). ![]() We use Biological, Chemical and Mechanical indicators to monitor our sterilization process. Instruments which can not be heat sterilized, are immersed in 2% glutaraldehyde for 10 hours to cold sterilize. Then the instruments are rinsed, dried, placed in self sealing sterilization pouches and sterilized in the autoclave. Following this pre-disinfection step the instruments are transferred to an ultrasonic cleaner for another 15 minutes. Patient debris and fluids are removed by placing the instruments in 3.2% glutaraldehyde for 40 minutes. We use Rapid Steam Autoclave at 275º F(35psi), for 15-20 minutesįirst, the instruments are prepared for the sterilization process. Sterilization refers to the use of a physical or chemical procedure to destroy all forms of microorganisms, including the highly resistant spores. ![]() Sterilization and disinfection are the basic steps in instrument processing and surface asepsis. Our office uses state of the art sterilization to ensure patient safety.
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